Part:BBa_K1059066:Design

GFP-LVA under RNA guardian control

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 854
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 709
  

Source

The GFP-LVA sequence is from part 145280, but we change the RBS. And we add K1509004 between B0015 and GFP-LVA, add k1509001 before GFP-LVA.